Guidelines for sending material for HbP analysis

The general indications for HbP analysis are:

For thalassemia and HbE carriers: slight or moderate microcytic hypochromic indices, with normal or slightly lower Hb values without iron deficiency or not improving after iron therapy.
For HbS, C en D carriers: in about 2/3 of HbS carriers only slightly abnormal indices are present. For these cases only the family history or the ethnical origin may provide an indication (African, Asiatic, or Mediterranean origin). Also Northern-Europeans may be carriers of abnormal hemoglobin or thalassemia (a-, b-thalassemia and HbD).

For a routine analysis send 10cc of fresh EDTA (Complexon) blood by fast mail every day of the week to the LUMC O&O building, Einthovenweg 20, Leiden. No appointment is needed but a thoroughly filled in request form is essential. The preliminary diagnosis based upon hematological and biochemical results can be given by e-mail or by telephone after approximately 1 week. The formal report including the DNA results will be sent to you by mail in about 3 months, after completion of the DNA analysis. In case of priority (hydrops foetalis or pre-operative HbS diagnosis) a result can be requested by telephone one working day after the sample has been delivered.

Yours sincerely,

C.L. Harteveld
Hemoglobinopathieën Laboratorium
Afd. Humane en Klinische Genetica
Leids Universitair Medisch Centrum

Einthovenweg 20
P.O.Box 9600, 2300 RC Leiden, Netherlands
Tel.: +31(0)71-5269800
E-mail: C.L.Harteveld@lumc.nl